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KMID : 0387919950060020106
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Korean Journal of Cytopathology
1995 Volume.6 No. 2 p.106 ~ p.115
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Immunocytochemical Characteristics of the Short-term Cultured Mesothelial Cells @AU 전호종, 이미자, 이미숙, 정유경, 이영미, 최형호
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전호종
이미자/이미숙/전유경/이영미/최형호
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Abstract
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Reactive human mesothelial cells were examined by immunocytochemical stain with intermediate filaments(cytokeratin [CK1, CK7, CK8, CK18, CD19], vimentin, desmin, actin), epithelial membrane antigen, carcinoembryonic antigen(CEA), MHC class
ll
antigen(HLA-DR), LeuM-1(CD15). a1-antitrypsin(ACT). a1-antichymotrypsin(ACHT), CD68(KP-1) and FcrRlll(CD16). The mesothelial cells were isolated from patients with liver cirrhosis and pleural effusion, and short-term cultured in RPMl 1640
media
containing 10% heat inactivated fetal calf serum and 1% identical supernatant fluid of the patients' transuates.
@ES The results obtained are as follows.
@EN 1. The cultured-reactive mesothelial cells were positive for the protein of cytoskeleton such as cytokeratin and vimentin but negative for desmin and actin. The resting mesothelial cells showed positive reactions for cytokeratin, but
negative
for vimentin, desmin and actin,
2. The primary antibodies to the cytokeratin were strongly reactive for CK1, CK8 and CK18 but negative for CK7 and CK19 in both reactive and resting mesothelial cells.
3. Resting mesothelial cells showed negative reactions for CEA, but strong positive reactions in cultured-reactive mesothelial cells.
4. The markers for the monocytes/histiocytes(CD11b, CD14, CD16, CD68, lysozyme and a1-antitrypsin and a1-antichymotrypsin) were nonreactive in resting mesothelial cells, but lysozyme and a1-antitrypsin were weakly reactive in
reactive
and proliferative mesothelial cells.
5. MHC Class ll molecule(HLA-DR antigen) was negative in both resting and reactive mesothelial cells.
These results suggest that the short-term cultured, reactive mesothelial cells show a newly aberrant expression of the vimentin and carcino-embryonic antigen. The reason of the aberrant expression of the intermediate filament and
oncofetal antigen in reactive and proliferative mesothelial cells should be further evaluated.
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KEYWORD
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